S5 (ATCC® HB-9255)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  /  Disease: Astrocytoma,sarcoma,sarcoma

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease Astrocytoma,sarcoma,sarcoma
Applications
The antibody reacts with a glycoprotein (gp120) of relative molecular weight 120000 daltons.
Genes Expressed
immunoglobulin; monoclonal antibody; against a human astrocytoma cell line; gp120 glycoprotein
Comments
Animals were immunized with the SK-MG-17 human astrocytoma cell line.
The antibody reacts with a glycoprotein (gp120) of relative molecular weight 120000 daltons.
The antigen is expressed on cultured human fibroblasts and sarcomas.
In normal tissues, S5 antigen expression is limited to visceral smooth muscle.
In a survey of tumors, S5 staining was limited to a subset of leiomyosarcomas and the staining was generally weaker than that with normal smooth muscle.
Complete Growth Medium RPMI 1640 medium with non-essential amino acids, 90%; fetal bovine serum, 10%
Complete Growth Medium
Culture medium: RPMI 1640 with non-essential amino acids, 90%; fetal bovine
serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
culture medium, 90%; DMSO, 10%
Isotype Secretes an IgG1 mouse monoclonal antibody.
Name of Depositor Sloan-Kettering Institute for Cancer Research
References

Rettig WJ, et al. Cell-surface glycoproteins of human sarcomas. US Patent 5,059,523 dated Oct 22 1991

Rettig WJ, et al. Differential expression of cell surface antigens and glial fibrillary acidic protein in human astrocytoma subsets. Cancer Res. 46: 6406-6412, 1986. PubMed: 2877731

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Rettig WJ, et al. Cell-surface glycoproteins of human sarcomas. US Patent 5,059,523 dated Oct 22 1991

Rettig WJ, et al. Differential expression of cell surface antigens and glial fibrillary acidic protein in human astrocytoma subsets. Cancer Res. 46: 6406-6412, 1986. PubMed: 2877731