OVB-3 [OVB3] (ATCC® HB-9147)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension, suspension
Biosafety Level 1
Applications
The antibody cross-reacts with various normal and neoplastic tissues.
Storage Conditions liquid nitrogen vapor phase
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human ovarian carcinoma
Comments
Animals were immunized with OVCAR-3 cells (see NIH:OVCAR-3, ATCC HTB-161).
Spleen cells were fused with NS-1 myeloma cells.
The antibody cross-reacts with various normal and neoplastic tissues.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Isotype IgG2a and 2b , IgG2a; IgG2b
Name of Depositor The United States of America
References

Willingham MC, et al. Pseudomonas exotoxin coupled to a monoclonal antibody against ovarian cancer inhibits the growth of human ovarian cancer cells in a mouse model. Proc. Natl. Acad. Sci. USA 84: 2474-2478, 1987. PubMed: 3104916

Pearson JW, et al. Enhanced therapeutic efficacy of an immunotoxin in combination with chemotherapy against an intraperitoneal human tumor xenograft in athymic mice. Cancer Res. 49: 4990-4995, 1989. PubMed: 2504482

Fitzgerald DJ, et al. Monoclonal antibody against ovarian cancer cells (OVB-3). US Patent 4,806,494 dated Feb 21 1989

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Willingham MC, et al. Pseudomonas exotoxin coupled to a monoclonal antibody against ovarian cancer inhibits the growth of human ovarian cancer cells in a mouse model. Proc. Natl. Acad. Sci. USA 84: 2474-2478, 1987. PubMed: 3104916

Pearson JW, et al. Enhanced therapeutic efficacy of an immunotoxin in combination with chemotherapy against an intraperitoneal human tumor xenograft in athymic mice. Cancer Res. 49: 4990-4995, 1989. PubMed: 2504482

Fitzgerald DJ, et al. Monoclonal antibody against ovarian cancer cells (OVB-3). US Patent 4,806,494 dated Feb 21 1989