mAb64 (ATCC® HB-8987)

Organism: Rattus norvegicus (B cell); Rattus norvegicus (myeloma), rat (B cell); rat (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Rattus norvegicus (B cell); Rattus norvegicus (myeloma), rat (B cell); rat (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Strain

Strain:  Lou (B cell); Lou (myeloma)

Applications
The antibody is specific for the alpha subunit of AChR and does not react with the main immunogenic region of AChR.
Derivation
Animals were immunized subcutaneously with purified, intact bovine calf acetylcholine receptor.
Spleen cells were fused with Y3 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against acetylcholine receptor (AChR) alpha subunit
Cellular Products
immunoglobulin; monoclonal antibody; against acetylcholine receptor (AChR) alpha subunit
Comments
Animals were immunized subcutaneously with purified, intact bovine calf acetylcholine receptor.
Spleen cells were fused with Y3 myeloma cells.
The antibody is specific for the alpha subunit of AChR and does not react with the main immunogenic region of AChR.
The antibody a has low affinity for S. aureus protein A.
Complete Growth Medium Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.01 mg/ml insulin, 0.01 mg/ml transferrin, 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype IgG2a
Name of Depositor Salk Institute for Biological Studies
References

Lindstrom JM. Assays for myasthenia gravis. US Patent 4,789,640 dated Dec 6 1988

Tzartos S, et al. Characteristics of monoclonal antibodies to denatured Torpedo and to native calf acetylcholine receptors: species, subunit and region specificity. J. Neuroimmunol. 10: 235-253, 1986. PubMed: 3484485

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Lindstrom JM. Assays for myasthenia gravis. US Patent 4,789,640 dated Dec 6 1988

Tzartos S, et al. Characteristics of monoclonal antibodies to denatured Torpedo and to native calf acetylcholine receptors: species, subunit and region specificity. J. Neuroimmunol. 10: 235-253, 1986. PubMed: 3484485