MH-SVM26 [SVM-26] (ATCC® HB-8872)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody reacts specifically with HIV-1 p24 and does not cross-react with p24 from HTLV-I or HTLV-II.
Derivation
Animals were immunized with lysates of purified HIV-1.
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against HTLV-III (HIV-1, human immunodeficiency virus - 1) p24 (24000 dalton protein)
Cellular Products
immunoglobulin; monoclonal antibody; against HTLV-III (HIV-1, human immunodeficiency virus - 1) p24 (24000 dalton protein)
Comments
Animals were immunized with lysates of purified HIV-1.
Spleen cells were fused with NS-1 myeloma cells.
The antibody reacts specifically with HIV-1 p24 and does not cross-react with p24 from HTLV-I or HTLV-II.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 2 mM L-glutamine, and 1 mM sodium pyruvate, 80%; fetal bovine serum, 20%
Complete Growth Medium
Culture Medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 2 mM
L-glutamine, 1 mM sodium pyruvate, 80%; fetal bovine serum, 20%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG1; kappa light chain
Name of Depositor Akzo Nobel N.V.
References

Sarngadharan MG, et al. Monoclonal antibodies for binding HTLV-III proteins, and cell lines for their production. US Patent 4,843,011 dated Jun 27 1989

Basic Documentation
References

Sarngadharan MG, et al. Monoclonal antibodies for binding HTLV-III proteins, and cell lines for their production. US Patent 4,843,011 dated Jun 27 1989