KC-4G3 [KC-4(IgG3)] (ATCC® HB-8709)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  /  Disease: Adenocarcinoma

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease Adenocarcinoma
Applications
The antibody reacts with an antigen present on solid tumors (98 of 104 tested) and cross-reacts with minimal cross reaction with paraffin embedded (14 of 92) or frozen (1 of 33) normal tissues.
An antibody with the same specificity but of the IgM class is available (ATCC HB-8710).
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human carcinoma cells
Cellular Products
immunoglobulin; monoclonal antibody; against human carcinoma cells
Comments
Animals were immunized with prostatic adenocarcinoma cells.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with an antigen present on solid tumors (98 of 104 tested) and cross-reacts with minimal cross reaction with paraffin embedded (14 of 92) or frozen (1 of 33) normal tissues.
The antigen is found in the cytoplasm as a 490000 dalton molecule, and in the membrane and cytoplasm as a 438000 dalton molecule.
An antibody with the same specificity but of the IgM class is available (ATCC HB-8710).
Complete Growth Medium Modified Dulbecco's medium with 0.1 mM hypoxanthine and 0.015 mM thymidine; fetal bovine serum, 15%
Complete Growth Medium
Culture Medium: Modified Dulbecco's medium (see below)with 0.1 mM hypoxanthine
and 0.015 mM thymidine, 85%; fetal bovine serum, 15%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
fetal bovine serum, 90%; DMSO, 10%
Isotype IgG3
Name of Depositor Coulter Corporation
References

Kortright KH, Hofheinz DE. Monoclonal antibody to a human carcinoma tumor associated antigen. US Patent 4,708,930 dated Nov 24 1987

Basic Documentation
References

Kortright KH, Hofheinz DE. Monoclonal antibody to a human carcinoma tumor associated antigen. US Patent 4,708,930 dated Nov 24 1987