TA 99 (ATCC® HB-8704)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Permits and Restrictions

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
This cell line produces a monoclonal antibody which can be used as a control for AS 33 (see ATCC HB-8779).
Genes Expressed
immunoglobulin; monoclonal antibody
Cellular Products
immunoglobulin; monoclonal antibody
Comments
This cell line produces a monoclonal antibody which can be used as a control for AS 33 (see ATCC HB-8779).
Complete Growth Medium RPMI 1640 medium with 2 mM L-glutamine, 1% nonessential amino acids, 100 units/ml penicillin and 0.1 mg/ml streptomycin, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
culture medium, 90%; DMSO, 10%
Isotype IgG2a; kappa light chain
Name of Depositor Sloan-Kettering Institute for Cancer Research
References

Welt S, et al. Method of imaging colorectal carcinoma lesion and composition for use therein. US Patent 5,160,723 dated Nov 3 1992

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Restrictions

The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the cells subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with the Office of Technology Development, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10065. Contact Tingting Zhang-Kharas, Direct Phone: 646-888-1083, Reception: 646-888-1080, Email: zhangkht@mskcc.org

References

Welt S, et al. Method of imaging colorectal carcinoma lesion and composition for use therein. US Patent 5,160,723 dated Nov 3 1992