TA 99 (ATCC® HB-8704)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Permits and Restrictions

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
This cell line produces a monoclonal antibody which can be used as a control for AS 33 (see ATCC HB-8779).
Genes Expressed
immunoglobulin; monoclonal antibody
Cellular Products
immunoglobulin; monoclonal antibody
Comments
This cell line produces a monoclonal antibody which can be used as a control for AS 33 (see ATCC HB-8779).
Complete Growth Medium RPMI 1640 medium with 2 mM L-glutamine, 1% nonessential amino acids, 100 units/ml penicillin and 0.1 mg/ml streptomycin, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
culture medium, 90%; DMSO, 10%
Isotype IgG2a; kappa light chain
Name of Depositor Sloan-Kettering Institute for Cancer Research
U.S. Patent Number
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.
References

Welt S, et al. Method of imaging colorectal carcinoma lesion and composition for use therein. US Patent 5,160,723 dated Nov 3 1992

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Restrictions

The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the cells subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with the Office of Technology Development, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10065. Contact email: otd@mskcc.org

References

Welt S, et al. Method of imaging colorectal carcinoma lesion and composition for use therein. US Patent 5,160,723 dated Nov 3 1992