gamma3-11.1 (ATCC® HB-8700)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Permits and Restrictions

View Permits

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Derivation
Spleen cells were fused with P3X63Ag8.U1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human gamma interferon
Cellular Products
immunoglobulin; monoclonal antibody; against human gamma interferon
Tumorigenic Yes
Effects
Yes, forms ascites in BALB/c mice
Comments
Animals were immunized with a synthetic peptide (NH2-Lys-Arg-Lys-Arg- Ser-Gln-Met-Leu-Phe-Arg-Gly-Arg-Arg-Ala-Ser-Gln-COOH) conjugated to thyroglobulin.
Spleen cells were fused with P3X63Ag8.U1 myeloma cells.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Complete Growth Medium
Culture Medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain
1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium
pyruvate, 90%; fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG1
Name of Depositor Takeda Chem. Ind., Ltd.
References

Ichimori Y, et al. Novel peptide and use thereof. US Patent 4,681,848 dated Jul 21 1987

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Ichimori Y, et al. Novel peptide and use thereof. US Patent 4,681,848 dated Jul 21 1987