Lym-2 (ATCC® HB-8613)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Permits and Restrictions

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

Strain:  BALB/c (B cell); BALB/c (myeloma)

Applications
The antibody stains a majority of human B cell derived tumors, but does not react with solid tumors.
Derivation
Spleen cells were fused with NS-1 myeloma cells.
The antibody stains a majority of human B cell derived tumors, but does not react with solid tumors.
Genes Expressed
immunoglobulin; monoclonal antibody; against chronic lymphocytic leukemia (CLL) cells
Cellular Products
immunoglobulin; monoclonal antibody; against chronic lymphocytic leukemia (CLL) cells
Comments
Animals were immunized with CLL cells.
Spleen cells were fused with NS-1 myeloma cells.
The antibody stains a majority of human B cell derived tumors, but does not react with solid tumors.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype IgG1
Name of Depositor Northwestern University
U.S. Patent Number
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.
References

Epstein AL. Murine hybridoma Lym-2 and diagnostic antibody produced thereby. US Patent 4,724,212 dated Feb 9 1988

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Epstein AL. Murine hybridoma Lym-2 and diagnostic antibody produced thereby. US Patent 4,724,212 dated Feb 9 1988