Mab 126 (ATCC® HB-8568)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  /  Disease: Neuroblastoma,Neuroblastoma, Carcinoma,Adenocarcinoma

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease Neuroblastoma,Neuroblastoma, Carcinoma,Adenocarcinoma
Applications
The antibody reacts strongly with neuroblastoma and melanoma cells, but does not react with lymphomas, sarcomas and most carcinomas.
Derivation
Spleen cells were fused with P3X63Ag8 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against ganglioside GD2
Cellular Products
immunoglobulin; monoclonal antibody; against ganglioside GD2
Tumorigenic Yes
Effects
Yes, forms ascites in BALB/c mice
Comments
Animals were immunized with neuroblastoma cells (LAN-1).
Spleen cells were fused with P3X63Ag8 myeloma cells.
Ganglioside GD2 is strongly expressed on cells of tumors of neuroectodermal origin (especially neuroblastoma and melanoma cells).
The antibody reacts strongly with neuroblastoma and melanoma cells, but does not react with lymphomas, sarcomas and most carcinomas.
Faint reactivity was reported for small cell carcinomas, adenocarcinomas of the adrenal cortex, gliomas and normal neural and muscle tissue.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgM
Name of Depositor Scripps Clinic and Research Foundation
References

Reisfeld RA. Monoclonal antibody directed to human ganglioside GD2. US Patent 4,675,287 dated Jun 23 1987

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Reisfeld RA. Monoclonal antibody directed to human ganglioside GD2. US Patent 4,675,287 dated Jun 23 1987