579 (ATCC® HB-8506)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Permits and Restrictions

View Permits

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody reacts with EGF receptors, blocks the binding of EGF to the receptor, and inhibits the growth of A-431 tumor cells in vitro.
The antibody binds to a single class of receptor sites on A-431, HeLa and human fibroblasts.
Derivation
Animals were immunized with partially purified EGF receptors from A-431 cells.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against epidermal growth factor (EGF) receptor
Cellular Products
immunoglobulin; monoclonal antibody; against epidermal growth factor (EGF) receptor
Tumorigenic Yes
Effects
Yes, forms ascites in pristane primed BALB/cJ mice
Comments
Animals were immunized with partially purified EGF receptors from A-431 cells.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with EGF receptors, blocks the binding of EGF to the receptor, and inhibits the growth of A-431 tumor cells in vitro.
The antibody binds to a single class of receptor sites on A-431, HeLa and human fibroblasts.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG
Name of Depositor Regents of the University of California
References

Mendelsohn J, et al. Hybrid cell lines that produce monoclonal antibodies to epidermal growth factor receptor. US Patent 4,943,533 dated Jul 24 1990

Sato JD, et al. Biological effects in vitro of monoclonal antibodies to human epidermal growth factor receptors. Mol. Biol. Med. 1: 511-529, 1983. PubMed: 6094961

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Mendelsohn J, et al. Hybrid cell lines that produce monoclonal antibodies to epidermal growth factor receptor. US Patent 4,943,533 dated Jul 24 1990

Sato JD, et al. Biological effects in vitro of monoclonal antibodies to human epidermal growth factor receptors. Mol. Biol. Med. 1: 511-529, 1983. PubMed: 6094961