J5-1 (ATCC® HB-8297)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody reacts with endotoxins from Gram negative bacteria, and inhibits endotoxin induced mitogenesis of B cells.
The antibody is protective in mice against Pseudomonas aeruginosa burn infection.
Derivation
Animals were immunized with purified E. coli J5 endotoxin core.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against Escherichia coli J5 endotoxin core
Cellular Products
immunoglobulin; monoclonal antibody; against Escherichia coli J5 endotoxin core
Tumorigenic Yes
Effects
Yes, forms ascites in pristane primed BALB/c mice
Comments
Animals were immunized with purified E. coli J5 endotoxin core.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with endotoxins from Gram negative bacteria, and inhibits endotoxin induced mitogenesis of B cells.
The antibody is protective in mice against Pseudomonas aeruginosa burn infection.
The epitope recognized by J5-1 appears to be distinct from that recognized by J5-2 (ATCC HB-8298).
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
RPMI 1640 medium with non-essential amino acids, 1 mM sodium pyruvate and 2 mM L-glutamine, 72.5%; fetal bovine serum, 20%; DMSO, 7.5%
Isotype IgM
Name of Depositor Centocor, Inc.
References

Pollack M, Hunter KW. Monoclonal antibodies reactive with endotoxin core. US Patent 5,057,598 dated Oct 15 1991

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Pollack M, Hunter KW. Monoclonal antibodies reactive with endotoxin core. US Patent 5,057,598 dated Oct 15 1991