2G8.D6 (ATCC® HB-8190)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against o-phosphotyrosine and proteins which contain phosphotyrosine (tyrosine phosphate)
Cellular Products
immunoglobulin; monoclonal antibody; against o-phosphotyrosine and proteins which contain phosphotyrosine (tyrosine phosphate)
Tumorigenic Yes
Effects
Yes, forms ascites in pristane primed BALB/c mice
Comments
Animals were immunized with p-azobenzylphosphonate covalently linked to a carrier protein.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Complete Growth Medium Iscove's modified Dulbecco's medium, 90%; fetal bovine serum, 10%
Complete Growth Medium
Culture Medium: Iscove's modified Dulbecco's medium, 90%; fetal bovine serum,
10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Culture Conditions
Temperature: 37.0°C
Isotype IgG1; kappa light chain
Name of Depositor Massachusetts Institute of Technology
U.S. Patent Number
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.
References

Eisen HN, et al. Production and use of monoclonal antibodies to phosphotyrosine-containing proteins. US Patent 4,543,439 dated Sep 24 1985

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Eisen HN, et al. Production and use of monoclonal antibodies to phosphotyrosine-containing proteins. US Patent 4,543,439 dated Sep 24 1985