2G8.D6 (ATCC® HB-8190™) Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) / Cell Type: hybridoma: B lymphocyte General Information Characteristics Culture Method Specifications History Documentation Print Email Share Share this product Facebook Twitter Google+ Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) Cell Type hybridoma: B lymphocyte Product Format frozen Morphology lymphoblast Culture Properties suspension Biosafety Level 1 Derivation Spleen cells were fused with Sp2/0-Ag14 myeloma cells. Genes Expressed immunoglobulin; monoclonal antibody; against o-phosphotyrosine and proteins which contain phosphotyrosine (tyrosine phosphate) Tumorigenic Yes Comments Animals were immunized with p-azobenzylphosphonate covalently linked to a carrier protein.Spleen cells were fused with Sp2/0-Ag14 myeloma cells. Complete Growth Medium Iscove's modified Dulbecco's medium, 90%; fetal bovine serum, 10% Subculturing Medium Renewal: Every 2 to 3 daysCultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. Culture Conditions Temperature: 37.0°C Isotype IgG1; kappa light chain Name of Depositor Massachusetts Institute of Technology References Eisen HN, et al. Production and use of monoclonal antibodies to phosphotyrosine-containing proteins. US Patent 4,543,439 dated Sep 24 1985 Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Basic Documentation Product Sheet Certificate of Analysis MSDS References Eisen HN, et al. Production and use of monoclonal antibodies to phosphotyrosine-containing proteins. US Patent 4,543,439 dated Sep 24 1985