9F12 (ATCC® HB-8177)

Organism: human (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism human (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Derivation
Human peripheral blood mononuclear cells (enriched for B cells) were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against tetanus toxin
Cellular Products
immunoglobulin; monoclonal antibody; against tetanus toxin
Comments
Human peripheral blood mononuclear cells (enriched for B cells) were fused with P3X63Ag8.653 myeloma cells.
Complete Growth Medium RPMI 1640 medium with 2 mM L-glutamine, 1 mM sodium pyruvate, 5 mcg/ml insulin and 5 mcg/ml transferrin, 85%; fetal bovine serum, 15%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Culture Conditions
Temperature: 37.0°C
Isotype IgG1
Name of Depositor University of Rochester Medical Center
References

Gigliotti F, Insel RA. Protective human hybridoma antibody to tetanus toxin. J. Clin. Invest. 70: 1306-1309, 1982. PubMed: 7174795

Insel RA. Human monoclonal antibodies against bacterial toxins. US Patent 4,689,299 dated Aug 25 1987

Gigliotti F, et al. Reproducible production of protective human monoclonal antibodies by fusion of peripheral blood lymphocytes with a mouse myeloma cell line. J. Infect. Dis. 149: 43-47, 1984. PubMed: 6607295

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Gigliotti F, Insel RA. Protective human hybridoma antibody to tetanus toxin. J. Clin. Invest. 70: 1306-1309, 1982. PubMed: 7174795

Insel RA. Human monoclonal antibodies against bacterial toxins. US Patent 4,689,299 dated Aug 25 1987

Gigliotti F, et al. Reproducible production of protective human monoclonal antibodies by fusion of peripheral blood lymphocytes with a mouse myeloma cell line. J. Infect. Dis. 149: 43-47, 1984. PubMed: 6607295