E6(2)2 (ATCC® HB-8172)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Permits and Restrictions

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody reacts with B6 vitamers.
It binds to both pyridoxine and pyridoxamine with similar affinities.
Since the product binds an essential nutrient, the medium must be changed frequently or the cells will die.
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against vitamin B6
Comments
Animals were immunized with phosphopyridoxyl reduced DNA binding proteins.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with B6 vitamers.
It binds to both pyridoxine and pyridoxamine with similar affinities.
Since the product binds an essential nutrient, the medium must be changed frequently or the cells will die.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose, and 0.1 mM non-essential amino acids, 70%; NCTC 109 medium, 10%; fetal bovine serum, 20%
Complete Growth Medium
CULTURE MEDIUM: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 0.1 mM
non-essential amino acids, 70%; NCTC 109 medium, 10%; fetal bovine serum, 20%.
Subculturing
Medium Renewal: 3 times per week
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
fetal bovine serum, 95%; DMSO, 5%
Isotype IgG1
Name of Depositor Research Corporation
References

Cidlowski JA, Viceps-Madore D. Monoclonal antibodies to vitamin B.sub.6 and immunoassay method. US Patent 4,465,776 dated Aug 14 1984

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Cidlowski JA, Viceps-Madore D. Monoclonal antibodies to vitamin B.sub.6 and immunoassay method. US Patent 4,465,776 dated Aug 14 1984