10F7MN (ATCC® HB-8162)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Strain

Strain:  Biozzi High Responder (B cell); BALB/c (myeloma)

Derivation
Animals were immunized with type O, Rh- human erythrocytes and purified glycophorin A.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against glycophorin A type M and type N
Cellular Products
immunoglobulin; monoclonal antibody; against glycophorin A type M and type N
Tumorigenic Yes
Effects
Yes, forms ascites in immunosuppressed BALB/c mice
Comments
Animals were immunized with type O, Rh- human erythrocytes and purified glycophorin A.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: 2 to 3 times per week
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
culture medium, 82.5%; fetal bovine serum, 10%; DMSO, 7.5%
Culture Conditions
Temperature: 37.0°C
Isotype IgG1; kappa light chain
Name of Depositor Lawrence Livermore National Lab
References

Bigbee WL, et al. Monoclonal antibodies specific for the M-and N-forms of human glycophorin A. Mol. Immunol. 20: 1353-1362, 1983. PubMed: 6197636

Bigbee WL, et al. Binding specificities of eight monoclonal antibodies to human glycophorin A. Studies with McM, and MkEn(UK) variant human erythrocytes and M- and MNv-type chimpanzee erythrocytes. J. Immunol. 133: 3149-3155, 1984. PubMed: 6491283

Bigbee WL, et al. Cell lines for the production of monoclonal antibodies to human glycophorin A. US Patent 4,767,710 dated Aug 30 1988

Basic Documentation
References

Bigbee WL, et al. Monoclonal antibodies specific for the M-and N-forms of human glycophorin A. Mol. Immunol. 20: 1353-1362, 1983. PubMed: 6197636

Bigbee WL, et al. Binding specificities of eight monoclonal antibodies to human glycophorin A. Studies with McM, and MkEn(UK) variant human erythrocytes and M- and MNv-type chimpanzee erythrocytes. J. Immunol. 133: 3149-3155, 1984. PubMed: 6491283

Bigbee WL, et al. Cell lines for the production of monoclonal antibodies to human glycophorin A. US Patent 4,767,710 dated Aug 30 1988