10F7MN (ATCC® HB-8162)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Strain

Strain:  Biozzi High Responder (B cell); BALB/c (myeloma)

Derivation
Animals were immunized with type O, Rh- human erythrocytes and purified glycophorin A.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against glycophorin A type M and type N
Cellular Products
immunoglobulin; monoclonal antibody; against glycophorin A type M and type N
Tumorigenic Yes
Effects
Yes, forms ascites in immunosuppressed BALB/c mice
Comments
Animals were immunized with type O, Rh- human erythrocytes and purified glycophorin A.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: 2 to 3 times per week
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
culture medium, 82.5%; fetal bovine serum, 10%; DMSO, 7.5%
Culture Conditions
Temperature: 37.0°C
Isotype IgG1; kappa light chain
Name of Depositor Lawrence Livermore National Lab
References

Bigbee WL, et al. Monoclonal antibodies specific for the M-and N-forms of human glycophorin A. Mol. Immunol. 20: 1353-1362, 1983. PubMed: 6197636

Bigbee WL, et al. Binding specificities of eight monoclonal antibodies to human glycophorin A. Studies with McM, and MkEn(UK) variant human erythrocytes and M- and MNv-type chimpanzee erythrocytes. J. Immunol. 133: 3149-3155, 1984. PubMed: 6491283

Bigbee WL, et al. Cell lines for the production of monoclonal antibodies to human glycophorin A. US Patent 4,767,710 dated Aug 30 1988

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Bigbee WL, et al. Monoclonal antibodies specific for the M-and N-forms of human glycophorin A. Mol. Immunol. 20: 1353-1362, 1983. PubMed: 6197636

Bigbee WL, et al. Binding specificities of eight monoclonal antibodies to human glycophorin A. Studies with McM, and MkEn(UK) variant human erythrocytes and M- and MNv-type chimpanzee erythrocytes. J. Immunol. 133: 3149-3155, 1984. PubMed: 6491283

Bigbee WL, et al. Cell lines for the production of monoclonal antibodies to human glycophorin A. US Patent 4,767,710 dated Aug 30 1988