WFL3C6.1 [3C6] (ATCC® HB-8157)

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody reacts with the LEW RT1.A class I molecule.
The antibody cross-reacts with human HLA A3.
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against LEW RT1.A and HLA A3; cytotoxic
Cellular Products
immunoglobulin; monoclonal antibody; against LEW RT1.A and HLA A3; cytotoxic
Comments
Animals were immunized with lymphoid cells from Lewis (LEW) rats.
Spleen cells were fused with NS-1 myeloma cells.
The antibody reacts with the LEW RT1.A class I molecule.
The antibody cross-reacts with human HLA A3.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Complete Growth Medium
Culture Medium: RPMI medium 1640, 90%; fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgM
Name of Depositor Brigham and Women's Hospital
References

Carpenter CB, et al. Monoclonal antibodies for human tissue cross-matching. US Patent 4,517,289 dated May 14 1985

Basic Documentation
References

Carpenter CB, et al. Monoclonal antibodies for human tissue cross-matching. US Patent 4,517,289 dated May 14 1985