3Pt12B8 (ATCC® HB-8136)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  /  Disease: Leukemia

Permits and Restrictions

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease Leukemia
Applications
The antibody has been used to prevent graft versus host disease (GVHD).
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against T12 (120000 dalton) antigen on human T cells (T lymphocyte); against CD6
Cellular Products
immunoglobulin; monoclonal antibody; against T12 (120000 dalton) antigen on human T cells (T lymphocyte); against CD6
Tumorigenic Yes
Effects
Yes, forms ascites in pristane primed BALB/cJ mice
Comments
Animals were immunized with human T chronic lymphocytic leukemia cells.
Spleen cells were fused with NS-1 myeloma cells.
The antibody has been used to prevent graft versus host disease (GVHD).
Complete Growth Medium Modified Dulbecco's medium; horse serum, 10%; fetal bovine serum, 10%
Complete Growth Medium
Culture Medium: Modified Dulbecco's medium (see below), 90%; horse serum, 10%.
DME w/HEPES (10mM) 100 mL
L-Glutamine (100x) 2 mL
Solution I 1 mL
Non-essential amino acids (100x) 1 mL
NCTC 135 10 mL
Horse Serum 12 mL
Solution I (100x)
1. 1320 mg oxalacetic acid (100 mM, MW 132).
2. 80 mg crystalline bovine insulin (20 units/mL, 25 units/mg).
Add 1 and 2, stir at 37°C. Add Na pyruvate 550 mg (50 mM, FW 110). Bring up to
100 mL with distilled water. Stir at 37°C until solution dissolves. Filter,
aliquot and store frozen.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgM
Name of Depositor Sidney Farber Cancer Institute
References

Reinherz EL, Schlossman SF. Monoclonal antibody. US Patent 4,443,427 dated Apr 17 1984

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Reinherz EL, Schlossman SF. Monoclonal antibody. US Patent 4,443,427 dated Apr 17 1984