3Pt12B8 (ATCC® HB-8136)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  /  Disease: Leukemia

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease Leukemia
Applications
The antibody has been used to prevent graft versus host disease (GVHD).
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against T12 (120000 dalton) antigen on human T cells (T lymphocyte); against CD6
Cellular Products
immunoglobulin; monoclonal antibody; against T12 (120000 dalton) antigen on human T cells (T lymphocyte); against CD6
Tumorigenic Yes
Effects
Yes, forms ascites in pristane primed BALB/cJ mice
Comments
Animals were immunized with human T chronic lymphocytic leukemia cells.
Spleen cells were fused with NS-1 myeloma cells.
The antibody has been used to prevent graft versus host disease (GVHD).
Complete Growth Medium Modified Dulbecco's medium; horse serum, 10%; fetal bovine serum, 10%
Complete Growth Medium
Culture Medium: Modified Dulbecco's medium (see below), 90%; horse serum, 10%.
DME w/HEPES (10mM) 100 mL
L-Glutamine (100x) 2 mL
Solution I 1 mL
Non-essential amino acids (100x) 1 mL
NCTC 135 10 mL
Horse Serum 12 mL
Solution I (100x)
1. 1320 mg oxalacetic acid (100 mM, MW 132).
2. 80 mg crystalline bovine insulin (20 units/mL, 25 units/mg).
Add 1 and 2, stir at 37°C. Add Na pyruvate 550 mg (50 mM, FW 110). Bring up to
100 mL with distilled water. Stir at 37°C until solution dissolves. Filter,
aliquot and store frozen.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgM
Name of Depositor Sidney Farber Cancer Institute
References

Reinherz EL, Schlossman SF. Monoclonal antibody. US Patent 4,443,427 dated Apr 17 1984

Basic Documentation
References

Reinherz EL, Schlossman SF. Monoclonal antibody. US Patent 4,443,427 dated Apr 17 1984