VD-10 (ATCC® HB-68)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody reacts with a 250000 dalton polypeptide that is part of the saxitoxin binding sodium channel protein in native electroplex membrane.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Animals were immunized with purified saxitoxin binding component of electric eel (E. electricus) electroplex membrane.
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against saxitoxin binding component of electroplex membrane
Cellular Products
immunoglobulin; monoclonal antibody; against saxitoxin binding component of electroplex membrane
Comments
Animals were immunized with purified saxitoxin binding component of electric eel (E. electricus) electroplex membrane.
Spleen cells were fused with NS-1 myeloma cells.
The antibody reacts with a 250000 dalton polypeptide that is part of the saxitoxin binding sodium channel protein in native electroplex membrane.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium RPMI 1640 medium, 80%; fetal bovine serum, 20%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG1
Name of Depositor LC Fritz, JP Brockes
References

Moore HP, et al. Isolation and characterization of a monoclonal antibody against the saxitoxin-binding component from the electric organ of the eel Electrophorus electricus. Proc. Natl. Acad. Sci. USA 79: 1673-1677, 1982. PubMed: 6280195

Basic Documentation
References

Moore HP, et al. Isolation and characterization of a monoclonal antibody against the saxitoxin-binding component from the electric organ of the eel Electrophorus electricus. Proc. Natl. Acad. Sci. USA 79: 1673-1677, 1982. PubMed: 6280195