20H2 (ATCC® HB-323)

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties mixed: adherent and suspension, The cells are rather adherent in nature, and grow best if maintained in suspension culture plates (e.g., Corning Catalog number 430591).
Biosafety Level 1
Strain

Strain: Wistar (B cell); BALB/c (myeloma)

Applications
The antibody reacts with CD1.1, and cross-reacts to the same extent with CD1.2.
The antibody performs well in flow cytometry applications, and is also useful in immunoprecipitation applications.
Derivation
Spleen cells were fused with Sp2/0-Ag14 cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse CD1.1
Cellular Products
immunoglobulin; monoclonal antibody; against mouse CD1.1
Comments
Animals were immunized with C57BL/6 thymocytes, boosted three times with the RBL-CD1.1 rat cell line (CD1.1-transfected), boosted once with BALB/cJ thymocytes and finally with the CR1-CD1.1 human cell line (CD1.1-transfected).
Spleen cells were fused with Sp2/0-Ag14 cells.
The antibody reacts with CD1.1, and cross-reacts to the same extent with CD1.2.
The antibody blocks recognition of CD1.1 by T cells in vitro and in vivo.
The antibody performs well in flow cytometry applications, and is also useful in immunoprecipitation applications.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation of the suspension with subsequent resuspension in fresh medium. Add medium as the cell density increases.
Maintain cultures at cell concentrations between 4 X 10(5) and 8 X 10(5) viable cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype IgG1; kappa light chain
Name of Depositor A Bendelac
References

Roark JH, et al. CD1.1 expression by mouse antigen-presenting cells and marginal zone B cells. J. Immunol. 160: 3121-3127, 1998. PubMed: 9531266

Park SH, et al. Tissue-specific recognition of mouse CD1 molecules. J. Immunol. 160: 3128-3134, 1998. PubMed: 9531267

Park SH, et al. Innate and adaptive functions of the CD1 pathway of antigen presentation. Semin. Immunol. 10: 391-398, 1998. PubMed: 9799714

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Roark JH, et al. CD1.1 expression by mouse antigen-presenting cells and marginal zone B cells. J. Immunol. 160: 3121-3127, 1998. PubMed: 9531266

Park SH, et al. Tissue-specific recognition of mouse CD1 molecules. J. Immunol. 160: 3128-3134, 1998. PubMed: 9531267

Park SH, et al. Innate and adaptive functions of the CD1 pathway of antigen presentation. Semin. Immunol. 10: 391-398, 1998. PubMed: 9799714