DREG56 (ATCC® HB-300)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human L-selectin (CD62L, LECAM, LAM-1, Leu8)
Comments
Animals were immunized with supernatant from phorbol myristic acid activated human peripheral blood leukocytes.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody blocks lymphocyte adhesion to high enothelial venules of peripheral lymph nodes.
Complete Growth Medium RPMI 1640 medium with 4.5 g/L glucose,15 mM HEPES, 2 mM L-glutamine, 0.01 mM nonessential amino acids and 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Isotype IgG1
Name of Depositor EC Butcher
References

Kishimoto TK, et al. Identification of a human peripheral lymph node homing receptor: a rapidly down-regulated adhesion molecule. Proc. Natl. Acad. Sci. USA 87: 2244-2248, 1990. PubMed: 2179952

Basic Documentation
References

Kishimoto TK, et al. Identification of a human peripheral lymph node homing receptor: a rapidly down-regulated adhesion molecule. Proc. Natl. Acad. Sci. USA 87: 2244-2248, 1990. PubMed: 2179952