FIB21 (ATCC® HB-295)

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  /  Disease: Lymphoma

Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease Lymphoma
Strain

Strain: Wistar (B cell); BALB/c (myeloma)

Tissue: B lymphocyte; hybridoma

Morphology: lymphoblast

Depositors: E.C. Butcher; D. Andrew

Products: immunoglobulin; monoclonal antibody; against murine b7 integrin

FreezeMedium: Culture medium, 95%; DMSO, 5%

FluidRenewal: Every 2 to 3 days

SubCulturing: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/mL and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/mL.

Growth Properties: suspension

Comments: Animals were immunized with cells of the TK1 murine T cell lymphoma. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with human and mouse b7 integrin. It partially blocks lymphocyte binding to MAdCAM-1, and a4b7 integrin binding to VCAM-1 and fibronectin. It is useful for both immunofluorescence and immunohistological staining. NOTE: a feeder layer of mouse peritoneal macrophages or X-irradiated mouse fibroblasts (see ATCC 56-X, irradiated STO cells) must be used in order to successfully recover these cells from the frozen state.

References:

          RF33063: Berlin C et al. Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1. Cell 74: 185-195, 1993 PubMed: 93327426

          RF33868: Andrew DP et al. Distinct but overlapping epitopes are involved in a4b7 - mediated adhesion to vascular cell adhesion molecule-1, mucosal addressin-1, fibronectin, and lymphocyte aggregation. J. Immunol. 153: 3847-3861, 1994 PubMed: 95015875

Applications
The antibody reacts with human and mouse beta 7 integrin.
It is useful for both immunofluorescence and immunohistological staining.
NOTE: a feeder layer of mouse peritoneal macrophages or X-irradiated mouse fibroblasts (see ATCC 56-X, irradiated STO cells) must be used in order to successfully recover these cells from the frozen state.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against murine beta 7 integrin
Cellular Products
immunoglobulin; monoclonal antibody; against murine beta 7 integrin
Comments
Animals were immunized with cells of the TK1 murine T cell lymphoma.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with human and mouse beta 7 integrin.
It partially blocks lymphocyte binding to MAdCAM-1, and alpha 4, beta 7 integrin binding to VCAM-1 and fibronectin.
It is useful for both immunofluorescence and immunohistological staining.
NOTE: a feeder layer of mouse peritoneal macrophages or X-irradiated mouse fibroblasts (see ATCC 56-X, irradiated STO cells) must be used in order to successfully recover these cells from the frozen state.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 0.05 mM 2-mercaptoethanol

Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype IgG2a
Name of Depositor EC Butcher, DP Andrew
References

Berlin C, et al. Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1. Cell 74: 185-195, 1993. PubMed: 7687523

Andrew DP, et al. Distinct but overlapping epitopes are involved in alpha 4 beta 7- mediated adhesion to vascular cell adhesion molecule-1, mucosal addressin-1, fibronectin, and lymphocyte aggregation. J. Immunol. 153: 3847-3861, 1994. PubMed: 7523506

Basic Documentation
References

Berlin C, et al. Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1. Cell 74: 185-195, 1993. PubMed: 7687523

Andrew DP, et al. Distinct but overlapping epitopes are involved in alpha 4 beta 7- mediated adhesion to vascular cell adhesion molecule-1, mucosal addressin-1, fibronectin, and lymphocyte aggregation. J. Immunol. 153: 3847-3861, 1994. PubMed: 7523506