CC29 (ATCC® HB-269)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody is specific for an antigen on bovine T cells that is analogous to the human CD5 antigen.
Derivation
Spleen cells were fused with NS1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against bovine CD5 (BoCD5)
Cellular Products
immunoglobulin; monoclonal antibody; against bovine CD5 (BoCD5)
Comments
Animals were immunized with neonatal bovine thymocytes.
Spleen cells were fused with NS1 myeloma cells.
The antibody is specific for an antigen on bovine T cells that is analogous to the human CD5 antigen.
The antigen is expressed on CD2 positive T cells, WC1 positive T cells and a subset of B cells.
This antibody was included in the panel of the International Workshop on Bovine, Sheep and Goat Leukocyte Differentiation Antigens in 1989.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Complete Growth Medium
Culture Medium: RPMI medium 1640, 90%; fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Isotype IgG1
Name of Depositor CJ Howard
References

Howard CJ, et al. In vivo depletion of BoT4 (CD4) and of non-T4/T8 lymphocyte subsets in cattle with monoclonal antibodies. Eur. J. Immunol. 19: 757-764, 1989. PubMed: 2567247

Howard CJ, et al. Two monoclonal antibodies (CC17, CC29) recognizing an antigen (Bo5) on bovine T lymphocytes, analogous to human CD5. Vet. Immunol. Immunopathol. 19: 127-139, 1988. PubMed: 3055661

Oldham G, Howard CJ. Suppression of bovine lymphocyte responses to mitogens following in vivo and in vitro treatment with dexamethasone. Vet. Immunol. Immunopathol. 30: 161-177, 1992. PubMed: 1534431

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Howard CJ, et al. In vivo depletion of BoT4 (CD4) and of non-T4/T8 lymphocyte subsets in cattle with monoclonal antibodies. Eur. J. Immunol. 19: 757-764, 1989. PubMed: 2567247

Howard CJ, et al. Two monoclonal antibodies (CC17, CC29) recognizing an antigen (Bo5) on bovine T lymphocytes, analogous to human CD5. Vet. Immunol. Immunopathol. 19: 127-139, 1988. PubMed: 3055661

Oldham G, Howard CJ. Suppression of bovine lymphocyte responses to mitogens following in vivo and in vitro treatment with dexamethasone. Vet. Immunol. Immunopathol. 30: 161-177, 1992. PubMed: 1534431