HS-21 (subclone 1H3) (ATCC® HB-255)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody reacts with an antigen on the acrosomal cap of mammalian sperm.
It is useful in immunofluorescent assays to evaluate acrosomal status in sperm samples.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against mammalian sperm acrosomal vesicle
Cellular Products
immunoglobulin; monoclonal antibody; against mammalian sperm acrosomal vesicle
Comments
Animals were immunized with human sperm.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with an antigen on the acrosomal cap of mammalian sperm.
It is useful in immunofluorescent assays to evaluate acrosomal status in sperm samples.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/l glucose, 90%; fetal bovine serum, 10%
Complete Growth Medium
CULTURE MEDIUM: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%;
fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Isotype IgG1
Name of Depositor KB Bechtol
References

Wolf DP, et al. Acrosomal status evaluation in human ejaculated sperm with monoclonal antibodies. Biol. Reprod. 32: 1157-1162, 1985. PubMed: 3926014

Basic Documentation
References

Wolf DP, et al. Acrosomal status evaluation in human ejaculated sperm with monoclonal antibodies. Biol. Reprod. 32: 1157-1162, 1985. PubMed: 3926014