17aba (ATCC® HB-248)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  /  Disease: Leukemia

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease Leukemia
Applications
The antibody reacts with human CD11b, and blocks all known CD11b dependent activities in vitro.
The antibody cross-reacts with CD11b from rat and canine cells.
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human CD11b
Cellular Products
immunoglobulin; monoclonal antibody; against human CD11b
Comments
Animals were immunized with human acute monocytic leukemia cells (FAM M6).
Spleen cells were fused with NS-1 myeloma cells.
The antibody reacts with human CD11b, and blocks all known CD11b dependent activities in vitro.
The antibody cross-reacts with CD11b from rat and canine cells.
Complete Growth Medium The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
  • heat-inactivated gamma globulin free horse serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere

Complete Growth Medium
Culture Medium: Modified Dulbecco's medium (see below), 90%; Gamma
globulin-free horse serum, 10%.
DME medium with 4.5 g/L glucose 100 mL
L-Glutamine (100x) 2 mL
Solution I 1 mL
Hypoxanthine (10 mM) - Thymidine (1.6 mM) 1 mL
NCTC 135 10 mL
Gamma Globulin-Free Horse Serum (inactivated) 12 mL
Solution I (100x)
1. 1320 mg oxalacetic acid (100 mM, MW 132).
2. 80 mg crystalline bovine insulin (20 units/mL, 25 units/mg).
Add 1 and 2, stir at 37°C. Add Na pyruvate 550 mg (50 mM, FW 110). Bring up to
100 mL with distilled water. Stir at 37°C until solution dissolves. Filter,
aliquot and store frozen.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgM; kappa light chain
Name of Depositor RF Todd
References

. . J. Inflamm. 13: 495-505, 1989.

Dana N, et al. Two functional domains in the phagocyte membrane glycoprotein Mo1 identified with monoclonal antibodies. J. Immunol. 137: 3259-3263, 1986. PubMed: 2430017

Mulligan MS, et al. Roles of beta 2 integrins of rat neutrophils in complement- and oxygen radical-mediated acute inflammatory injury. J. Immunol. 148: 1847-1857, 1992. PubMed: 1347308

Basic Documentation
References

. . J. Inflamm. 13: 495-505, 1989.

Dana N, et al. Two functional domains in the phagocyte membrane glycoprotein Mo1 identified with monoclonal antibodies. J. Immunol. 137: 3259-3263, 1986. PubMed: 2430017

Mulligan MS, et al. Roles of beta 2 integrins of rat neutrophils in complement- and oxygen radical-mediated acute inflammatory injury. J. Immunol. 148: 1847-1857, 1992. PubMed: 1347308