CIA-E-7.12 (ATCC® HB-236)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody reacts with human epsilon heavy chain.
Derivation
Lymph node cells were fused with S194/5.XXO.BU.1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human epsilon immunoglobulin heavy chain (IgE)
Cellular Products
immunoglobulin; monoclonal antibody; against human epsilon immunoglobulin heavy chain (IgE)
Tumorigenic Yes
Effects
Yes, forms ascites in pristane-primed BALB/c mice
Comments
Animals were immunized with a human IgE paraprotein (PS).
Lymph node cells were fused with S194/5.XXO.BU.1 myeloma cells.
The antibody reacts with human epsilon heavy chain.
It has a very high affinity (9 X 10 exp-10 m/L) for an epitope located between amino acids 218 and 301 (CH2 domain) of human epsilon.
It does not bind to protein A, but functions well in histamine release assays.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG1; kappa light chain
Name of Depositor A Saxon
References

Macy E, et al. Enhanced ELISA: how to measure less than 10 picograms of a specific protein (immunoglobulin) in less than 8 hours. FASEB J. 2: 3003-3009, 1988. PubMed: 3263291

Hassner A, Saxon A. Isotype-specific human suppressor T cells for IgE synthesis activated by IgE-anti-IgE immune complexes. J. Immunol. 132: 2844-2849, 1984. PubMed: 6233363

Generally, the first i.p. passage yields mostly adherent tumor cells and little ascites, but serial passage of the ascites cell in additional BALB/c mice will yield large amounts of ascites that may contain up to 20 mg/ml of the antibody. For monoclonal antibody production in ascites, cells should be injected (10 to 20 million cells) intraperitoneally (i.p.) in pristane-primed BALB/c mice.

Basic Documentation
References

Macy E, et al. Enhanced ELISA: how to measure less than 10 picograms of a specific protein (immunoglobulin) in less than 8 hours. FASEB J. 2: 3003-3009, 1988. PubMed: 3263291

Hassner A, Saxon A. Isotype-specific human suppressor T cells for IgE synthesis activated by IgE-anti-IgE immune complexes. J. Immunol. 132: 2844-2849, 1984. PubMed: 6233363

Generally, the first i.p. passage yields mostly adherent tumor cells and little ascites, but serial passage of the ascites cell in additional BALB/c mice will yield large amounts of ascites that may contain up to 20 mg/ml of the antibody. For monoclonal antibody production in ascites, cells should be injected (10 to 20 million cells) intraperitoneally (i.p.) in pristane-primed BALB/c mice.