35.1 (ATCC® HB-222)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody reacts with the human T cell surface protein Tp50 (CD2).
Derivation
Spleen cells were fused with NSI/1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human CD2
Cellular Products
immunoglobulin; monoclonal antibody; against human CD2
Comments
Animals were immunized with human T lymphocytes that had been activated with phorbol myristic acid (PMA).
Spleen cells were fused with NSI/1 myeloma cells.
The antibody reacts with the human T cell surface protein Tp50 (CD2).
The antibody inhibits T cell proliferative responses and cytotoxicity, but does not inhibit antibody mediated cellular cytotoxicity.
Complete Growth Medium RPMI 1640 medium with 0.05 mM 2-mercaptoethanol and 2 mM L-glutamine, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 5 X 10 exp5 cells/ml.
Isotype IgG2a
Name of Depositor PJ Martin
References

Martin PJ, et al. Identification and functional characterization of two distinct epitopes on the human T cell surface protein Tp50. J. Immunol. 131: 180-185, 1983. PubMed: 6190907

Basic Documentation
References

Martin PJ, et al. Identification and functional characterization of two distinct epitopes on the human T cell surface protein Tp50. J. Immunol. 131: 180-185, 1983. PubMed: 6190907