35.1 (ATCC® HB-222)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody reacts with the human T cell surface protein Tp50 (CD2).
Derivation
Spleen cells were fused with NSI/1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human CD2
Cellular Products
immunoglobulin; monoclonal antibody; against human CD2
Comments
Animals were immunized with human T lymphocytes that had been activated with phorbol myristic acid (PMA).
Spleen cells were fused with NSI/1 myeloma cells.
The antibody reacts with the human T cell surface protein Tp50 (CD2).
The antibody inhibits T cell proliferative responses and cytotoxicity, but does not inhibit antibody mediated cellular cytotoxicity.
Complete Growth Medium RPMI 1640 medium with 0.05 mM 2-mercaptoethanol and 2 mM L-glutamine, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 5 X 10 exp5 cells/ml.
Isotype IgG2a
Name of Depositor PJ Martin
References

Martin PJ, et al. Identification and functional characterization of two distinct epitopes on the human T cell surface protein Tp50. J. Immunol. 131: 180-185, 1983. PubMed: 6190907

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Martin PJ, et al. Identification and functional characterization of two distinct epitopes on the human T cell surface protein Tp50. J. Immunol. 131: 180-185, 1983. PubMed: 6190907