T3-3A1 [3A1] (ATCC® HB-2)

Organism: Mus musculus, mouse  /  Cell Type: hybridoma: B lymphocyte; somatic cell hybri  /  Disease: identifying T cell acute lymphoblastic leukemia.,identifying T cell acute lymphoblastic leukemia.

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Organism Mus musculus, mouse
Cell Type hybridoma: B lymphocyte; somatic cell hybri
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease identifying T cell acute lymphoblastic leukemia.,identifying T cell acute lymphoblastic leukemia.
Age adult
Strain

Strain:  BALB/c

Applications
The antibody recognizes a 40000 dalton cell surface antigen that is an early marker of the T cell lineage and may be useful for identifying T cell acute lymphoblastic leukemia.
This line is derived from the same fusion used to produce ATCC HB-21 and ATCC HB-22.
Storage Conditions liquid nitrogen vapor phase
Derivation
This line is derived from the same fusion used to produce ATCC HB-21 and ATCC HB-22.
Comments
The antibody recognizes a 40000 dalton cell surface antigen that is an early marker of the T cell lineage and may be useful for identifying T cell acute lymphoblastic leukemia.
This line is derived from the same fusion used to produce ATCC HB-21 and ATCC HB-22.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(5) viable cells/ml.
Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) cells/ml
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype mouse IgG1 kappa
Name of Depositor A Fauci, BF Haynes
References

Haynes BF, et al. Human lymphocyte antigens: production of a monoclonal antibody that defines functional thymus-derived lymphocyte subsets. Proc. Natl. Acad. Sci. USA 76: 5829-5833, 1979. PubMed: 316538

Eisenbarth GS, et al. Production of monoclonal antibodies reacting with peripheral blood mononuclear cell surface differentiation antigens. J. Immunol. 124: 1237-1244, 1980. PubMed: 6153676

Haynes BF, et al. Characterization of a monoclonal antibody that defines an immunoregulatory T cell subset for immunoglobulin synthesis in humans. Proc. Natl. Acad. Sci. USA 77: 2914-2918, 1980. PubMed: 6156457

Prieyl JA, LeBien TW. Interleukin 7 independent development of human B cells. Proc. Natl. Acad. Sci. USA 93: 10348-10353, 1996. PubMed: 8816803

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Haynes BF, et al. Human lymphocyte antigens: production of a monoclonal antibody that defines functional thymus-derived lymphocyte subsets. Proc. Natl. Acad. Sci. USA 76: 5829-5833, 1979. PubMed: 316538

Eisenbarth GS, et al. Production of monoclonal antibodies reacting with peripheral blood mononuclear cell surface differentiation antigens. J. Immunol. 124: 1237-1244, 1980. PubMed: 6153676

Haynes BF, et al. Characterization of a monoclonal antibody that defines an immunoregulatory T cell subset for immunoglobulin synthesis in humans. Proc. Natl. Acad. Sci. USA 77: 2914-2918, 1980. PubMed: 6156457

Prieyl JA, LeBien TW. Interleukin 7 independent development of human B cells. Proc. Natl. Acad. Sci. USA 93: 10348-10353, 1996. PubMed: 8816803