356-1 (ATCC® HB-181)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody reacts with a moderately conserved epitope on the JVB strain of CB4.
The antibody also reacts with myocardium from several species including mouse, guinea pig, chicken and rabbit.
Neutralizes many human isolates of CB4.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against coxsackievirus B4 (CB4); also reacts with heart muscle (myocardium); neutralizing
Tumorigenic Yes
Effects
Yes, in BALB/c mice
Comments
Animals were immunized with coxsackievirus B4 (JVB strain, ATCC VR 144).
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with a moderately conserved epitope on the JVB strain of CB4.
The antibody also reacts with myocardium from several species including mouse, guinea pig, chicken and rabbit.
Neutralizes many human isolates of CB4.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Complete Growth Medium
Culture Medium: Dulbecco's modified Eagle's medium (containing 1 mM sodium
pyruvate) with 4.5 g/L glucose, 90%; fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG2a; kappa light chain
Name of Depositor BS Prabhakar
References

Prabhaker BS, et al. High frequency of antigenic variants among naturally occurring human Coxsackie B4 virus isolates identified by monoclonal antibodies. Nature 300: 374-376, 1982. PubMed: 6183593

Prabhaker BS, et al. Detection of conserved and nonconserved epitopes on Coxsackievirus B4: frequency of antigenic change. Virology 146: 302-306, 1985. PubMed: 2413617

Ungar BL, et al. Enzyme immunoassay detection of immunoglobulin M and G antibodies to Cryptosporidium in immunocompetent and immunocompromised persons. J. Infect. Dis. 153: 570-578, 1986. PubMed: 3950440

Webb SR, et al. Neutralization epitope diversity of Coxsackievirus B4 isolates detected by monoclonal antibodies. J. Med. Virol. 20: 9-15, 1986. PubMed: 2428930

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Prabhaker BS, et al. High frequency of antigenic variants among naturally occurring human Coxsackie B4 virus isolates identified by monoclonal antibodies. Nature 300: 374-376, 1982. PubMed: 6183593

Prabhaker BS, et al. Detection of conserved and nonconserved epitopes on Coxsackievirus B4: frequency of antigenic change. Virology 146: 302-306, 1985. PubMed: 2413617

Ungar BL, et al. Enzyme immunoassay detection of immunoglobulin M and G antibodies to Cryptosporidium in immunocompetent and immunocompromised persons. J. Infect. Dis. 153: 570-578, 1986. PubMed: 3950440

Webb SR, et al. Neutralization epitope diversity of Coxsackievirus B4 isolates detected by monoclonal antibodies. J. Med. Virol. 20: 9-15, 1986. PubMed: 2428930