anti-IR-1 [aIR-1, alpha IR-1] (ATCC® HB-175)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody reacts strongly with insulin receptors from human placenta and IM-9 cells (ATCC CCL-159).
It reacts less efficiently with insulin receptors on human erythrocytes.
The antibody cross-reacts with the receptor for somatomedin C.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Animals were immunized with purified human placental insulin receptor.
Spleen cells were fused with FO myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human insulin receptor
Cellular Products
immunoglobulin; monoclonal antibody; against human insulin receptor
Comments
Animals were immunized with purified human placental insulin receptor.
Spleen cells were fused with FO myeloma cells.
The antibody reacts strongly with insulin receptors from human placenta and IM-9 cells (ATCC CCL-159).
It reacts less efficiently with insulin receptors on human erythrocytes.
The antibody cross-reacts with the receptor for somatomedin C.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Complete Growth Medium
Culture Medium: RPMI medium 1640, 90%; fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG1; kappa light chain
Name of Depositor S Jacobs, FC Kull
References

Kull FC Jr., et al. Monoclonal antibodies to receptors for insulin and somatomedin-C. J. Biol. Chem. 258: 6561-6566, 1983. PubMed: 6304046

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Kull FC Jr., et al. Monoclonal antibodies to receptors for insulin and somatomedin-C. J. Biol. Chem. 258: 6561-6566, 1983. PubMed: 6304046