B27M2 (ATCC® HB-165)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
Animals were immunized with human peripheral blood leukocytes (HLA phenotype A2,A28,B27,B44,Bw4,Cw2).
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen and lymph node cells were fused with Sp2/08A2 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against HLA B27, Bw47; cytotoxic
Cellular Products
immunoglobulin; monoclonal antibody; against HLA B27, Bw47; cytotoxic
Comments
Animals were immunized with human peripheral blood leukocytes (HLA phenotype A2,A28,B27,B44,Bw4,Cw2).
Spleen and lymph node cells were fused with Sp2/08A2 myeloma cells.
The antibody is cytotoxic for a subpopulation of HLA B27 positive cells and for all HLA Bw47 positive cells at an appropriate titer.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium Modified RPMI 1640 medium (see fact sheet), 90%; fetal bovine serum, 10%
Complete Growth Medium
Culture Medium: Modified RPMI 1640 medium (see below) with 50 uM
2-mercaptoethanol, 90%; fetal bovine serum, 10%.
RPMI 1640 100 mL
L-Glutamine (100x) 1 mL
Solution FM 1 mL
Non-essential amino acids (100x) 1 mL
Fetal Bovine Serum 12 mL
Solution FM (100x)
1. 1320 mg oxalacetic acid (100 mM, MW 132).
2. 100 mg crystalline bovine insulin (20 units/mL, 25 units/mg).
Add 1 and 2, stir at 37°C. Add Na pyruvate 450 mg (41 mM, FW 110). Bring up to
100 mL with distilled water. Stir at 37°C until solution dissolves. Filter,
aliquot and store frozen.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgM
Name of Depositor F Grumet
References

Grumet FC, et al. Monoclonal antibody (B27M2) subdividing HLA-B27. Hum. Immunol. 5: 61-72, 1982. PubMed: 6981637

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Grumet FC, et al. Monoclonal antibody (B27M2) subdividing HLA-B27. Hum. Immunol. 5: 61-72, 1982. PubMed: 6981637