76-2-11 (ATCC® HB-143)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody reacts with a 35000 dalton antigen from the surface of cytotoxic T cells.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against pig cytotoxic T cell; cytotoxic
Cellular Products
immunoglobulin; monoclonal antibody; against pig cytotoxic T cell; cytotoxic
Comments
Animals were immunized with thymocytes from dd miniature swine.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with a 35000 dalton antigen from the surface of cytotoxic T cells.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
  • Complete Growth Medium
    Culture Medium: Modified Dulbecco's medium (see below), 90%; fetal bovine
    serum, 10%.
    DME w/HEPES (10mM) 100 mL
    L-Glutamine (100x) 1 mL
    Solution I 1 mL
    Non-essential amino acids (100x) 1 mL
    NCTC 135 10 mL
    Fetal Bovine Serum 12 mL
    Solution I (100x)
    1. 1320 mg oxalacetic acid (100 mM, MW 132).
    2. 80 mg crystalline bovine insulin (20 units/mL, 25 units/mg).
    Add 1 and 2, stir at 37°C. Add Na pyruvate 550 mg (50 mM, FW 110). Bring up to
    100 mL with distilled water. Stir at 37°C until solution dissolves. Filter,
    aliquot and store frozen.
    Subculturing
    Medium Renewal: Every 2 to 3 days
    Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
    Isotype IgG2a; kappa light chain
    Name of Depositor JR Lunney
    References

    Pescovitz MD, et al. Preparation and characterization of monoclonal antibodies reactive with porcine PBL. J. Immunol. 133: 368-375, 1984. PubMed: 6609988

    Lunney JK, et al. Overview of the first international workshop to define swine leukocyte cluster of differentiation (CD) antigens. Vet. Immunol. Immunopathol. 43: 193-206, 1994. PubMed: 7856053

    Notice: Necessary PermitsPermits

    These permits may be required for shipping this product:

    • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
    Basic Documentation
    References

    Pescovitz MD, et al. Preparation and characterization of monoclonal antibodies reactive with porcine PBL. J. Immunol. 133: 368-375, 1984. PubMed: 6609988

    Lunney JK, et al. Overview of the first international workshop to define swine leukocyte cluster of differentiation (CD) antigens. Vet. Immunol. Immunopathol. 43: 193-206, 1994. PubMed: 7856053