74-11-10 (ATCC® HB-139™)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody cross-reacts with SLA ABa.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against SLA ABd (pig histocompatibility antigen); cytotoxic
Comments
Animals were immunized with dd miniature swine thymocytes.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody cross-reacts with SLA ABa.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
    • Subculturing
    Medium Renewal: Every 2 to 3 days
    Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
    Isotype IgG2b; kappa light chain
    Name of Depositor JR Lunney
    References

    Pescovitz MD, et al. Preparation and characterization of monoclonal antibodies reactive with porcine PBL. J. Immunol. 133: 368-375, 1984. PubMed: 6609988

    Permits Notice: Necessary Permits

    These permits may be required for shipping this product:

    • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
    Basic Documentation
    Product Sheet
    Product Sheet
    Certificate of Analysis
    Certificate of Analysis
    MSDS
    MSDS
    References

    Pescovitz MD, et al. Preparation and characterization of monoclonal antibodies reactive with porcine PBL. J. Immunol. 133: 368-375, 1984. PubMed: 6609988