2A2-6E10-1D8 [1D8] (ATCC® HB-12465)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Strain

Strain: BALB/c (B cell); BALB/c (myeloma)

Applications
Animals were immunized with stool-derived Norwalk virus.
The antibody is directed against an epitope of the 58 kDa antigen of Norwalk Virus.
The antibody specifically binds to Norwalk virus particles found in human stool specimens from naturally occurring Norwalk virus outbreaks.
The antibody reacts with the prototype 8FIIa strain of Norwalk virus in an enzyme immunoassay (EIA) for detecting Norwalk virus in stool specimens.
Derivation
Animals were immunized with stool-derived Norwalk virus.
Spleen cells were fused with Sp2/0 mouse myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against Norwalk Virus
Cellular Products
immunoglobulin; monoclonal antibody; against Norwalk Virus
Tumorigenic Yes
Effects
Yes, forms ascites in BALB/c mice
Comments
Animals were immunized with stool-derived Norwalk virus.
Spleen cells were fused with Sp2/0 mouse myeloma cells.
The antibody is directed against an epitope of the 58 kDa antigen of Norwalk Virus.
The antibody specifically binds to Norwalk virus particles found in human stool specimens from naturally occurring Norwalk virus outbreaks.
The antibody reacts with the prototype 8FIIa strain of Norwalk virus in an enzyme immunoassay (EIA) for detecting Norwalk virus in stool specimens.
No cross-reactions were seen in stools containing other caliciviruses or in stools containing rotaviruses, astroviruses, or enteric adenoviruses.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Attached cells may need to be shaken loose.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype IgG1
Name of Depositor University of Massachusetts Medical Center
References

Herrmann JE, Blacklow NR. Monoclonal antibodies for detecting Norwalk virus. US Patent 5,861,241 dated Jan 19 1999

Herrmann JE, et al. Monoclonal antibodies for detection of Norwalk virus antigen in stools. J. Clin. Microbiol. 33: 2511-2513, 1995. PubMed: 7494063

Brinker JP, et al. Detection of Norwalk virus and other genogroup 1 human caliciviruses by a monoclonal antibody, recombinant-antigen-based immunoglobulin M capture enzyme immunoassay. J. Clin. Microbiol. 36: 1064-1069, 1998. PubMed: 9542938

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Herrmann JE, Blacklow NR. Monoclonal antibodies for detecting Norwalk virus. US Patent 5,861,241 dated Jan 19 1999

Herrmann JE, et al. Monoclonal antibodies for detection of Norwalk virus antigen in stools. J. Clin. Microbiol. 33: 2511-2513, 1995. PubMed: 7494063

Brinker JP, et al. Detection of Norwalk virus and other genogroup 1 human caliciviruses by a monoclonal antibody, recombinant-antigen-based immunoglobulin M capture enzyme immunoassay. J. Clin. Microbiol. 36: 1064-1069, 1998. PubMed: 9542938