GAP 8.3 (ATCC® HB-12)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Strain

Strain:  BALB/c (B cell); BALB/c (myeloma)

Applications
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human leukocyte (monocytes, lymphocytes, granulocytes); against CD45
Comments
Mice were immunized with MOLT-4 cells a human T cell line (MOLT-4, ATCC CRL-1582).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody recognizes a 200000 dalton antigen analogous to the mouse T200 antigen and the rat common leukocyte antigen (it does not react with erythrocytes).
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Culture Conditions
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Isotype IgG2a; kappa light chain
Name of Depositor AE Berger
References

Berger AE, et al. A human leukocyte antigen identified by a monoclonal antibody. Hum. Immunol. 3: 231-245, 1981. PubMed: 6975769

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Berger AE, et al. A human leukocyte antigen identified by a monoclonal antibody. Hum. Immunol. 3: 231-245, 1981. PubMed: 6975769