HECA-452 (ATCC® HB-11485)

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Strain

Strain: Wistar

Applications
The antibody reacts with an antigen on skin homing memory T cells.
It binds a T cell carbohydrate ligand for vascular E-selectin.
Storage Conditions liquid nitrogen vapor phase
Genes Expressed
immunoglobulin; monoclonal antibody; against human cutaneous lymphocyte antigen (CLA)
Cellular Products
immunoglobulin; monoclonal antibody; against human cutaneous lymphocyte antigen (CLA)
Comments
The antibody reacts with an antigen on skin homing memory T cells.
It binds a T cell carbohydrate ligand for vascular E-selectin.
The antibody also recognizes sialyl Lewis x and sialyl Lewis a antigens and other carbohydrate ligands for human E-selectin.
Complete Growth Medium RPMI 1640 medium with 0.05 mM 2-mercaptoethanol and non essential amino acids, 90%; fetal bovine serum, 10%
Subculturing
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: culture medium, 92.5%; DMSO, 7.5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Isotype rat IgM
Name of Depositor Stanford University
References

Butcher EC, Picker LJ. Neutrophil LECAM-1 as indicator of neutrophil activation. US Patent 5,316,913 dated May 31 1994

Duijvestijn AM, et al. High endothelial differentiation in human lymphoid and inflammatory tissues defined by monoclonal antibody HECA-452. Am. J. Pathol. 130: 147-155, 1988. PubMed: 3276207

Berg EL, et al. The cutaneous lymphocyte antigen is a skin lymphocyte homing receptor for the vascular lectin endothelial cell-leukocyte adhesion molecule 1. J. Exp. Med. 174: 1461-1466, 1991. PubMed: 1720810

The antibody reacts with a 200-kd cell-surface glycoprotein present on a subset of peripheral blood T cells, including both CD4+ and CD8+ T cells.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Butcher EC, Picker LJ. Neutrophil LECAM-1 as indicator of neutrophil activation. US Patent 5,316,913 dated May 31 1994

Duijvestijn AM, et al. High endothelial differentiation in human lymphoid and inflammatory tissues defined by monoclonal antibody HECA-452. Am. J. Pathol. 130: 147-155, 1988. PubMed: 3276207

Berg EL, et al. The cutaneous lymphocyte antigen is a skin lymphocyte homing receptor for the vascular lectin endothelial cell-leukocyte adhesion molecule 1. J. Exp. Med. 174: 1461-1466, 1991. PubMed: 1720810

The antibody reacts with a 200-kd cell-surface glycoprotein present on a subset of peripheral blood T cells, including both CD4+ and CD8+ T cells.