10C4.1.3 (ATCC® HB-11029)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma:lymphoblast B lymphocyte; somatic cell hybri  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma:lymphoblast B lymphocyte; somatic cell hybri
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Strain

Strain: BALB/c (B cell); BALB/c (myeloma)

Applications
The antibody is specific for alpha v,beta 3 integrin.
It recognizes an epitope on alpha v,beta 3 integrin that is specific to human osteoclasts.
Storage Conditions liqid nitrogen vapor phase
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Derivation
Animals were immunized with purified alpha v,beta 3 integrin.
Spleen cells were fused with P3X63Ag8U.1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human alpha v,beta 3 integrin
Cellular Products
immunoglobulin; monoclonal antibody; against human alpha v,beta 3 integrin
Tumorigenic Yes
Effects
Yes, forms ascites in pristane primed BALB/c mice
Comments
Animals were immunized with purified alpha v,beta 3 integrin.
Spleen cells were fused with P3X63Ag8U.1 myeloma cells.
The antibody is specific for alpha v,beta 3 integrin.
It recognizes an epitope on alpha v,beta 3 integrin that is specific to human osteoclasts.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10(5) cells/ml. Do not allow the cell concentration to exceed 1 X 10(6) cells/ml.
Interval: Maintain between 1 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium supplemented with an additional 10% fetal bovine serum and 7.5% (v/v) DMSO
Storage temperature: liqid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype IgG1 kappa , IgG1; kappa light chain
Name of Depositor Genentech, Inc.
References

Kim KJ, et al. Antibody to osteoclast alphavbeta3 ntegrin. US Patent 5,578,704 dated Nov 26 1996

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Kim KJ, et al. Antibody to osteoclast alphavbeta3 ntegrin. US Patent 5,578,704 dated Nov 26 1996