20-8-4S (ATCC® HB-11)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Strain

Strain:  C3H (B cell); BALB/c (myeloma)

Applications
The antibody cross-reacts with H-2Kd, H-2Ks and H-2Kr.
Tested and found negative for ectromelia virus (mousepox).
Storage Conditions liquid nitrogen vapor phase
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against H-2 K b, D b; cytotoxic
Comments
Mice were immunized with spleen cells from C3H.SW mice.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody cross-reacts with H-2Kd, H-2Ks and H-2Kr.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
  • Subculturing
    Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 (5) viable cells/ml. Maintain cell density between 1 X 10 (5) and 1 X 10 (6) viable cells/ml.
    Medium Renewal: Every 2 to 3 days
    Cryopreservation
    Freeze medium: Complete growth medium, 95%; DMSO, 5%
    Storage temperature: liquid nitrogen vapor phase
    Culture Conditions
    Atmosphere: 5% CO2 in air recommended
    Temperature: 37.0°C
    Isotype IgG2a kappa
    Name of Depositor DH Sachs, K Ozato
    References

    Ozato K, Sachs DH. Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression. J. Immunol. 126: 317-321, 1981. PubMed: 6935293

    Notice: Necessary PermitsPermits

    These permits may be required for shipping this product:

    • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
    Basic Documentation
    References

    Ozato K, Sachs DH. Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression. J. Immunol. 126: 317-321, 1981. PubMed: 6935293