5c8 (ATCC® HB-10916)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Strain

Strain:  BALB/c (B cell); BALB/c (myeloma)

Storage Conditions liquid nitrogen vapor phase
Derivation
Spleen cells were fused with Sp2/0 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against T-B activating molecule T-BAM, ligand for CD40 (CD40L, CD154)
Cellular Products
immunoglobulin; monoclonal antibody; against T-B activating molecule T-BAM, ligand for CD40 (CD40L, CD154)
Comments
Animals were immunized with D1.1 cells (see ATCC CRL-10915).
Spleen cells were fused with Sp2/0 myeloma cells.
The antibody recognizes an antigen present on T cells that is important to the T cell mediated activation of B cells.
The antigen is a ligand for the CD40 molecule on the B cell surface.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml.
Interval: Maintain between 1 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype mouse IgG2a
Name of Depositor Trustees of Columbia University
References

Lederman S, et al. Murine monoclonal antibody (5c8) recognizes a human glycoprotein on the surface of T-lymphocytes, compositions containing same. US Patent 5,474,771 dated Dec 12 1995

Lederman S, et al. Methods for treating autoimmune diseases with 5C8-specific antibodies. US Patent 6,592,868 dated Jul 15 2003

. Protein recognized by an antibody that specifically binds an epitope that is specifically bound by monoclonal antibody 5c8. U.S. Patent 6,793,924 dated Sep 21 2004

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Lederman S, et al. Murine monoclonal antibody (5c8) recognizes a human glycoprotein on the surface of T-lymphocytes, compositions containing same. US Patent 5,474,771 dated Dec 12 1995

Lederman S, et al. Methods for treating autoimmune diseases with 5C8-specific antibodies. US Patent 6,592,868 dated Jul 15 2003

. Protein recognized by an antibody that specifically binds an epitope that is specifically bound by monoclonal antibody 5c8. U.S. Patent 6,793,924 dated Sep 21 2004