558-D 100, 10, 1 [558-D] (ATCC® HB-10894)

Organism: human (B cell); human x mouse (heterohybridoma)  /  Cell Type: hybridoma: B lymphocyte; Epstein-Barr virus (EBV) transforme  / 

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Organism human (B cell); human x mouse (heterohybridoma)
Cell Type hybridoma: B lymphocyte; Epstein-Barr virus (EBV) transforme
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2 Cells contain Human Immunodeficiency virus type 1 (HIV-1)
Age adult
Strain

Strain: BALB/c (myeloma)

Applications
It has broad HIV group specificity and reacts with the HTLV-IIIB, MN and SF-2 strains of HIV-1.
The antibody is specific for the CD4-binding domain of the gp120 protein of HIV-1; it inhibits CD4-rgp120 binding.
The antibody can be used in ELISA and neutralization assays.
Storage Conditions liquid nitrogen vapor phase
Derivation
Antibody producing clones were expanded and fused with SHM-D33 heterohybridoma cells (ATCC CRL-1668).
Clinical Data
Human peripheral blood mononuclear cells from HIV seropositive patients were transformed with Epstein-Barr virus (EBV).
Comments
Human peripheral blood mononuclear cells from HIV seropositive patients were transformed with Epstein-Barr virus (EBV).
Antibody producing clones were expanded and fused with SHM-D33 heterohybridoma cells (ATCC CRL-1668).
The antibody is specific for the CD4-binding domain of the gp120 protein of HIV-1; it inhibits CD4-rgp120 binding. It has broad HIV group specificity and reacts with the HTLV-IIIB, MN and SF-2 strains of HIV-1.
The antibody can be used in ELISA and neutralization assays.
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml. Maintain cell density between 1 X 10 exp5 and 2 X 10 exp6 viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Isotype IgG1 kappa
Name of Depositor New York University
References

Zolla-Pazner S, et al. Human monoclonal antibodies to the CD4-binding domain of HIV, uses thereof and synergistic neutralization of HIV. US Patent 6,241,986 dated Jun 5 2001

Wang H, et al. Human monoclonal and polyclonal anti-human immunodeficiency virus-1 antibodies share a common clonotypic specificity. Eur. J. Immunol. 22: 1749-1755, 1992. PubMed: 1378015

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Zolla-Pazner S, et al. Human monoclonal antibodies to the CD4-binding domain of HIV, uses thereof and synergistic neutralization of HIV. US Patent 6,241,986 dated Jun 5 2001

Wang H, et al. Human monoclonal and polyclonal anti-human immunodeficiency virus-1 antibodies share a common clonotypic specificity. Eur. J. Immunol. 22: 1749-1755, 1992. PubMed: 1378015