4E8C12 (ATCC® HB-10452)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
Animals were immunized with a rough strain of O157:H7 (Escherichia coli HA1, deficient in the O157 antigen).
The antibody is specific for E. coli O157:H7 and O26:H11 both of which are enterohemorrhagic.
It reacts with a protein antigen of 13000 daltons.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against an enterohemorrhagic strain of Escherichia coli (E. coli O157:H7 strain 932)
Cellular Products
immunoglobulin; monoclonal antibody; against an enterohemorrhagic strain of Escherichia coli (E. coli O157:H7 strain 932)
Tumorigenic Yes
Effects
Yes, forms ascites in pristane primed BALB/c mice
Comments
Animals were immunized with a rough strain of O157:H7 (Escherichia coli HA1, deficient in the O157 antigen).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody is specific for E. coli O157:H7 and O26:H11 both of which are enterohemorrhagic.
It reacts with a protein antigen of 13000 daltons.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 50 units/ml penicillin, 0.05 mg/ml streptomycin and 0.05 mg/ml gentamicin, 80%; fetal bovine serum, 20%
Complete Growth Medium

Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
culture medium, 90%; DMSO, 10%
Isotype Secretes a mouse IgG2a monoclonal antibody specific for enteropathogenic
Escherichia coli serotypes O157:H7 and O26:H11.
Name of Depositor Wisconsin Alumni Res. Fndn.
References

Doyle MP, Padhye N. Monoclonal antibody to enterohemorrhagic Escherichia coli 0157:H7 and 026:H11. US Patent 5,168,063 dated Dec 1 1992

Doyle MP, Padhye N. Monoclonal antibody to enterohemorrhagic Escherichia coli 0157:H7 and 026:H11 and method for detection. US Patent 5,354,661 dated Oct 11 1994

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Doyle MP, Padhye N. Monoclonal antibody to enterohemorrhagic Escherichia coli 0157:H7 and 026:H11. US Patent 5,168,063 dated Dec 1 1992

Doyle MP, Padhye N. Monoclonal antibody to enterohemorrhagic Escherichia coli 0157:H7 and 026:H11 and method for detection. US Patent 5,354,661 dated Oct 11 1994