4E8C12 (ATCC® HB-10452)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Animals were immunized with a rough strain of O157:H7 (Escherichia coli HA1, deficient in the O157 antigen).
The antibody is specific for E. coli O157:H7 and O26:H11 both of which are enterohemorrhagic.
It reacts with a protein antigen of 13000 daltons.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against an enterohemorrhagic strain of Escherichia coli (E. coli O157:H7 strain 932)
Cellular Products
immunoglobulin; monoclonal antibody; against an enterohemorrhagic strain of Escherichia coli (E. coli O157:H7 strain 932)
Tumorigenic Yes
Effects
Yes, forms ascites in pristane primed BALB/c mice
Comments
Animals were immunized with a rough strain of O157:H7 (Escherichia coli HA1, deficient in the O157 antigen).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody is specific for E. coli O157:H7 and O26:H11 both of which are enterohemorrhagic.
It reacts with a protein antigen of 13000 daltons.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 50 units/ml penicillin, 0.05 mg/ml streptomycin and 0.05 mg/ml gentamicin, 80%; fetal bovine serum, 20%
Complete Growth Medium

Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
culture medium, 90%; DMSO, 10%
Isotype Secretes a mouse IgG2a monoclonal antibody specific for enteropathogenic
Escherichia coli serotypes O157:H7 and O26:H11.
Name of Depositor Wisconsin Alumni Res. Fndn.
U.S. Patent Number
References

Doyle MP, Padhye N. Monoclonal antibody to enterohemorrhagic Escherichia coli 0157:H7 and 026:H11. US Patent 5,168,063 dated Dec 1 1992

Doyle MP, Padhye N. Monoclonal antibody to enterohemorrhagic Escherichia coli 0157:H7 and 026:H11 and method for detection. US Patent 5,354,661 dated Oct 11 1994

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Doyle MP, Padhye N. Monoclonal antibody to enterohemorrhagic Escherichia coli 0157:H7 and 026:H11. US Patent 5,168,063 dated Dec 1 1992

Doyle MP, Padhye N. Monoclonal antibody to enterohemorrhagic Escherichia coli 0157:H7 and 026:H11 and method for detection. US Patent 5,354,661 dated Oct 11 1994