ILB1-H67 (ATCC® HB-10222)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts specifically with IL-1 beta and does not react with IL-1 alpha or with acidic or basic fibroblast growth factor.
Derivation
Animals were immunized with purified recombinant human IL-1 beta (170500 dalton form).
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human interleukin 1 beta (interleukin-1 beta, IL-1 beta)
Cellular Products
immunoglobulin; monoclonal antibody; against human interleukin 1 beta (interleukin-1 beta, IL-1 beta)
Tumorigenic Yes
Effects
Yes, forms ascites in BALB/c mice
Comments
Animals were immunized with purified recombinant human IL-1 beta (170500 dalton form).
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts specifically with IL-1 beta and does not react with IL-1 alpha or with acidic or basic fibroblast growth factor.
It recognizes an epitope distinct from that bound by ILB1-H6 and ILB1-H34 (ATCC HB-10219 and ATCC HB-10221).
The affinity constant of ILB1-H67 was measured to be 4.8 X 10 exp10 liter/mol.
Complete Growth Medium The base medium for this cell line is HB101 medium (from Irvine Scientific). To make the complete growth medium, add fetal bovine serum to a final concentration of 7% Alternately, cells may be grown on ATCC Hybri-Care Medium Catalog No. 46-X as the base medium. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. It must be supplemented with 1.5 g/L sodium bicarbonate.
Complete Growth Medium
CULTURE MEDIUM: Dulbecco's modified Eagle's medium with 4 mM L-glutamine
adjusted to contain 1.5g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM sodium
pyruvate, 90%; fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
culture medium, 90%; DMSO, 10%
Isotype IgG2b; kappa light chain
Name of Depositor Syntex (USA), Inc.
U.S. Patent Number
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.
References

Allison AC, et al. Monoclonal antibodies for interleukin-1.beta. US Patent 4,935,343 dated Jun 19 1990

Kenney JS, et al. Monoclonal antibodies to human recombinant interleukin 1 (IL 1)beta: quantitation of IL 1 beta and inhibition of biological activity. J. Immunol. 138: 4236-4242, 1987. PubMed: 2438338

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Allison AC, et al. Monoclonal antibodies for interleukin-1.beta. US Patent 4,935,343 dated Jun 19 1990

Kenney JS, et al. Monoclonal antibodies to human recombinant interleukin 1 (IL 1)beta: quantitation of IL 1 beta and inhibition of biological activity. J. Immunol. 138: 4236-4242, 1987. PubMed: 2438338