ILB1-H67 (ATCC® HB-10222)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody reacts specifically with IL-1 beta and does not react with IL-1 alpha or with acidic or basic fibroblast growth factor.
Derivation
Animals were immunized with purified recombinant human IL-1 beta (170500 dalton form).
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human interleukin 1 beta (interleukin-1 beta, IL-1 beta)
Cellular Products
immunoglobulin; monoclonal antibody; against human interleukin 1 beta (interleukin-1 beta, IL-1 beta)
Tumorigenic Yes
Effects
Yes, forms ascites in BALB/c mice
Comments
Animals were immunized with purified recombinant human IL-1 beta (170500 dalton form).
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts specifically with IL-1 beta and does not react with IL-1 alpha or with acidic or basic fibroblast growth factor.
It recognizes an epitope distinct from that bound by ILB1-H6 and ILB1-H34 (ATCC HB-10219 and ATCC HB-10221).
The affinity constant of ILB1-H67 was measured to be 4.8 X 10 exp10 liter/mol.
Complete Growth Medium The base medium for this cell line is HB101 medium (from Irvine Scientific). To make the complete growth medium, add fetal bovine serum to a final concentration of 7% Alternately, cells may be grown on ATCC Hybri-Care Medium Catalog No. 46-X as the base medium. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. It must be supplemented with 1.5 g/L sodium bicarbonate.
Complete Growth Medium
CULTURE MEDIUM: Dulbecco's modified Eagle's medium with 4 mM L-glutamine
adjusted to contain 1.5g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM sodium
pyruvate, 90%; fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
culture medium, 90%; DMSO, 10%
Isotype IgG2b; kappa light chain
Name of Depositor Syntex (USA), Inc.
References

Allison AC, et al. Monoclonal antibodies for interleukin-1.beta. US Patent 4,935,343 dated Jun 19 1990

Kenney JS, et al. Monoclonal antibodies to human recombinant interleukin 1 (IL 1)beta: quantitation of IL 1 beta and inhibition of biological activity. J. Immunol. 138: 4236-4242, 1987. PubMed: 2438338

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Allison AC, et al. Monoclonal antibodies for interleukin-1.beta. US Patent 4,935,343 dated Jun 19 1990

Kenney JS, et al. Monoclonal antibodies to human recombinant interleukin 1 (IL 1)beta: quantitation of IL 1 beta and inhibition of biological activity. J. Immunol. 138: 4236-4242, 1987. PubMed: 2438338