1116NS-3d (ATCC® CRL-8019)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  /  Disease: Adenocarcinoma

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease Adenocarcinoma
Derivation
Spleen cells were fused with P3X63Ag8 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against carcinoembryonic antigen (CEA)
Cellular Products
immunoglobulin; monoclonal antibody; against carcinoembryonic antigen (CEA)
Comments
Animals were immunized with a human colonic adenocarcinoma (SW1116, ATCC CCL-233).
Spleen cells were fused with P3X63Ag8 myeloma cells.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgM
Name of Depositor Wistar Institute
References

Koprowski H, et al. Monoclonal hybridoma antibody specific for high molecular weight carcinoembryonic antigen. US Patent 4,349,528 dated Sep 14 1982

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Koprowski H, et al. Monoclonal hybridoma antibody specific for high molecular weight carcinoembryonic antigen. US Patent 4,349,528 dated Sep 14 1982