OKT 5 (ATCC® CRL-8016)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Derivation
Spleen cells were fused with P3X63Ag8.U1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human T cell (human cytotoxic/suppressor T lymphocyte)
Comments
Animals were immunized with human peripheral blood T lymphocytes.
Spleen cells were fused with P3X63Ag8.U1 myeloma cells.
Complete Growth Medium The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
  • Subculturing
    Medium Renewal: Every 2 to 3 days
    Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
    Cryopreservation
    culture medium 95%; DMSO, 5%
    Culture Conditions
    Temperature: 37.0°C
    Isotype IgG1
    Name of Depositor Ortho Pharmaceutical Corporation
    References

    Kung PC, Goldstein G. Monoclonal antibody to human cytotoxic and suppressor T cells and methods of preparing same. US Patent 4,364,932 dated Dec 21 1982

    Notice: Necessary PermitsPermits

    These permits may be required for shipping this product:

    • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
    Basic Documentation
    References

    Kung PC, Goldstein G. Monoclonal antibody to human cytotoxic and suppressor T cells and methods of preparing same. US Patent 4,364,932 dated Dec 21 1982