KIM185 (ATCC® CRL-2839)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma:lymphoblast B lymphocyte; somatic cell hybri  / 

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma:lymphoblast B lymphocyte; somatic cell hybri
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
KIM185 stimulates cell homotypic aggregation by a CD11 pathway in the JY human B lymphoblastoid cell line, and it induces the adherence of neutrophils to protein-coated plastic by a CD11b-dependent mechanism [PubMed: 7690325].
Storage Conditions liquid nitrogen vapor phase
Images
Derivation
Animals were immunized with CR3 (CD11b/CD18). Spleen cells were fused with Sp2/0 myeloma cells. KIM185 stimulates cell homotypic aggregation by a CD11 pathway in the JY human B lymphoblastoid cell line, and it induces the adherence of neutrophils to protein-coated plastic by a CD11b-dependent mechanism [PubMed: 7690325]. The antibody recognizes an epitope distinct from the KIM127 (ATCC CRL-2838) antibody.
Comments
Animals were immunized with CR3 (CD11b/CD18). Spleen cells were fused with Sp2/0 myeloma cells. KIM185 stimulates cell homotypic aggregation by a CD11 pathway in the JY human B lymphoblastoid cell line, and it induces the adherence of neutrophils to protein-coated plastic by a CD11b-dependent mechanism [PubMed: 7690325]. The antibody recognizes an epitope distinct from the KIM127 (ATCC CRL-2838) antibody.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Shake off attached cells and scrape if necessary. Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(4) to 1 X 10(5) viable cells/ml.
Interval: Maintain cultures at a cell concentration between 2.5 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium with 20% fetal bovine serum and 7.5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype mouse IgG1 kappa
Population Doubling Time 20 hours
Name of Depositor MK Robinson
Year of Origin 1989
References

Andrew D, et al. KIM185, a monoclonal antibody to CD18 which induces a change in the conformation of CD18 and promotes both LFA-1- and CR3-dependent adhesion. Eur. J. Immunol. 23: 2217-2222, 1993. PubMed: 7690325

Basic Documentation
Other Documentation
References

Andrew D, et al. KIM185, a monoclonal antibody to CD18 which induces a change in the conformation of CD18 and promotes both LFA-1- and CR3-dependent adhesion. Eur. J. Immunol. 23: 2217-2222, 1993. PubMed: 7690325