1G12 (ATCC® CRL-2827)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte; somatic cell hybri  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte; somatic cell hybri
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain CB.17
Storage Conditions liquid nitrogen vapor phase
Images
Derivation
Animals were immunized with T cells from 3A9 T cell receptor (TCR) transgenic mice. Spleen cells were fused with P3X63Ag8 myeloma cells.The antibody recognizes the 3A9 T cell receptor (TCR).
Comments
Animals were immunized with T cells from 3A9 T cell receptor (TCR) transgenic mice. Spleen cells were fused with P3X63Ag8 myeloma cells.
The antibody recognizes the 3A9 T cell receptor (TCR).
Complete Growth Medium Dulbecco's Modified Eagle's Medium with 4 mM L-glutamine that is modified by ATCC to contain 4.5 g/L glucose and 1.5 g/L sodium bicarbonates supplemented with 1.5 mM L-glutamine, 0.05 mM 2-mercaptoethanol, 10 mM HEPES, 116mg/L L-arginine HCl, 41 mg/L L-asparagine.H2O and 10% heat-inactivated fetal bovine serum
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 8 X 10(4) viable cells/ml.
Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium supplemented with an additional 10% heat-inactivated fetal bovine serum and 7.5% DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype IgG1 kappa
Population Doubling Time 10 hours
Name of Depositor ER Unanue
Year of Origin 1996
References

Van Parijs L, et al. The Fas/Fas ligand pathway and Bcl-2 regulate T cell responses to model self and foreign antigens. Immunity 8: 265-274, 1998. PubMed: 9492007

Peterson DA, et al. Quantitative analysis of the T cell repertoire that escapes negative selection. Immunity 11: 453-462, 1999. PubMed: 10549627

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Van Parijs L, et al. The Fas/Fas ligand pathway and Bcl-2 regulate T cell responses to model self and foreign antigens. Immunity 8: 265-274, 1998. PubMed: 9492007

Peterson DA, et al. Quantitative analysis of the T cell repertoire that escapes negative selection. Immunity 11: 453-462, 1999. PubMed: 10549627