1G12 (ATCC® CRL-2827)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte; somatic cell hybri  / 

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte; somatic cell hybri
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Strain CB.17
Storage Conditions liquid nitrogen vapor phase
Images
Derivation
Animals were immunized with T cells from 3A9 T cell receptor (TCR) transgenic mice. Spleen cells were fused with P3X63Ag8 myeloma cells.The antibody recognizes the 3A9 T cell receptor (TCR).
Comments
Animals were immunized with T cells from 3A9 T cell receptor (TCR) transgenic mice. Spleen cells were fused with P3X63Ag8 myeloma cells.
The antibody recognizes the 3A9 T cell receptor (TCR).
Complete Growth Medium Dulbecco's Modified Eagle's Medium with 4 mM L-glutamine that is modified by ATCC to contain 4.5 g/L glucose and 1.5 g/L sodium bicarbonates supplemented with 1.5 mM L-glutamine, 0.05 mM 2-mercaptoethanol, 10 mM HEPES, 116mg/L L-arginine HCl, 41 mg/L L-asparagine.H2O and 10% heat-inactivated fetal bovine serum
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 8 X 10(4) viable cells/ml.
Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium supplemented with an additional 10% heat-inactivated fetal bovine serum and 7.5% DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype IgG1 kappa
Population Doubling Time 10 hours
Name of Depositor ER Unanue
Year of Origin 1996
References

Van Parijs L, et al. The Fas/Fas ligand pathway and Bcl-2 regulate T cell responses to model self and foreign antigens. Immunity 8: 265-274, 1998. PubMed: 9492007

Peterson DA, et al. Quantitative analysis of the T cell repertoire that escapes negative selection. Immunity 11: 453-462, 1999. PubMed: 10549627

Basic Documentation
Other Documentation
References

Van Parijs L, et al. The Fas/Fas ligand pathway and Bcl-2 regulate T cell responses to model self and foreign antigens. Immunity 8: 265-274, 1998. PubMed: 9492007

Peterson DA, et al. Quantitative analysis of the T cell repertoire that escapes negative selection. Immunity 11: 453-462, 1999. PubMed: 10549627