LHR-74 (ATCC® CRL-2686)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Permits and Restrictions

View Permits

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibodies can be used in Western Blot, ELISA, immunocytochemistry, and immunohistochemistry assays of the human LH/hCG receptor.
Derivation
Spleen cells were fused with Sp2/0 mouse myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human luteinizing hormone/human chorionic gonadotropin (LH/hCG) receptor, extracellular domain
Cellular Products
immunoglobulin; monoclonal antibody; against human luteinizing hormone/human chorionic gonadotropin (LH/hCG) receptor, extracellular domain
Tumorigenic Yes
Effects
Yes, forms ascites in BALB/c mice
Comments
Animals were immunized with recombinant human luteinizing hormone/human chorionic gonadotropin (LH/hCG) receptor expressed in E. coli.
Spleen cells were fused with Sp2/0 mouse myeloma cells.
The antibody recognizes the extracellular domain (hormone binding domain) of the LH/hCG receptor, (amino acids domain 21-362).
The LHR-29 (ATCC CRL-2685), LHR-74 (ATCC CRL-2686) and LHR-1055 (ATCC CRL-2687) antibodies are additive. They recognize different epitopes.
The antibodies can be used in Western Blot, ELISA, immunocytochemistry, and immunohistochemistry assays of the human LH/hCG receptor.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 90%; heat-inactivated horse serum, 10%
Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml.
Maintain cell density between 2 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype IgG1; kappa light chain
Name of Depositor H Loosfelt, E Milgrom
References

Meduri G, et al. Luteinizing hormone/human chorionic gonadotropin receptors in breast cancer. Cancer Res. 57: 857-864, 1997. PubMed: 9041186

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Meduri G, et al. Luteinizing hormone/human chorionic gonadotropin receptors in breast cancer. Cancer Res. 57: 857-864, 1997. PubMed: 9041186