TSHR-R5T-34 (ATCC® CRL-2683)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody cross-reacts with rodent TSH receptor.
The antibodies can be used in Western Blot, ELISA, immunocytochemistry, and immunohistochemistry assays of the human TSH receptor.
Derivation
Spleen cells were fused with Sp2/0 mouse myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human thyroid stimulating hormone (TSH) receptor beta subunit, extracellular domain
Cellular Products
immunoglobulin; monoclonal antibody; against human thyroid stimulating hormone (TSH) receptor beta subunit, extracellular domain
Tumorigenic Yes
Effects
Yes, forms ascites in BALB/c mice
Comments
Animals were immunized with recombinant human thyroid stimulating hormone (TSH) receptor expressed in. E. coli.
Spleen cells were fused with Sp2/0 mouse myeloma cells.
The antibody recognizes the N-terminal extracellular domain (amino acids domain 357-389), beta subunit of the human thyroid stimulating hormone (TSH) receptor. It recognizes an extracellular epitope of the beta subunit anchored into the cellular membrane.
The antibody does not interfere with hormone binding and has no effect on the receptor mediated adenylate cyclase activity.
The antibody cross-reacts with rodent TSH receptor.
The TSHR-R5T-34 (ATCC CRL-2683) antibody recognizes a N-terminal extracellular epitope of the beta subunit while the TSHR-T3-365 (ATCC CRL-2684) antibody recognizes a C-terminal intracellular subunit of the beta subunit.
The antibodies can be used in Western Blot, ELISA, immunocytochemistry, and immunohistochemistry assays of the human TSH receptor.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 90%; heat-inactivated horse serum, 10%
Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Cryopreservation
Culture medium, 45%; heat-inactivated horse serum, 45%; DMSO, 10%.
Culture Conditions
Temperature: 37.0°C
Isotype IgG1; kappa light chain
Name of Depositor H Loosfelt, E Milgrom
References

Baratti-Elbaz C, et al. Internalization and recycling pathways of the thyrotropin receptor. Mol. Endocrinol. 13: 1751-1765, 1999. PubMed: 10517676

de Bernard S, et al. Sequential cleavage and excision of a segment of the thyrotropin receptor ectodomain. J. Biol. Chem. 274: 101-107, 1999. PubMed: 9867816

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Baratti-Elbaz C, et al. Internalization and recycling pathways of the thyrotropin receptor. Mol. Endocrinol. 13: 1751-1765, 1999. PubMed: 10517676

de Bernard S, et al. Sequential cleavage and excision of a segment of the thyrotropin receptor ectodomain. J. Biol. Chem. 274: 101-107, 1999. PubMed: 9867816