P-9 (ATCC® CRL-2590)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma:  / 

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma:
Product Format frozen
Morphology lymphoblast
Culture Properties suspension, with some loosely adherent cells
Biosafety Level 1
Applications
The antibody can be used for immunochemical and Western blot applications.
It is a useful tool for detecting and localizing the large subunit of mu-calpain.
The antibody reacts with the large subunit of human mu-calpain.
. It cross-reacts with the large subunit of rat, mouse and hamster mu-calpain.
Storage Conditions liquid nitrogen vapor phase
Derivation
Animals were immunized with mu-calpain isolated from human erythrocytes. Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells. The antibody reacts with the large subunit of human mu-calpain.. It cross-reacts with the large subunit of rat, mouse and hamster mu-calpain. The antibody does not cross-react with human placental m-calpain [PubMed: 8702541]. The antibody can be used for immunochemical and Western blot applications. It is a useful tool for detecting and localizing the large subunit of mu-calpain.
Comments
Animals were immunized with mu-calpain isolated from human erythrocytes. Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells. The antibody reacts with the large subunit of human mu-calpain.. It cross-reacts with the large subunit of rat, mouse and hamster mu-calpain. The antibody does not cross-react with human placental m-calpain [PubMed: 8702541]. The antibody can be used for immunochemical and Western blot applications. It is a useful tool for detecting and localizing the large subunit of mu-calpain.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10(5) viable cells/ml. Attached cells may be subcultured by scraping.
Interval: Maintain cell density between 2 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium supplemented with an additional 45% fetal bovine serum and 10% DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype kappa
Name of Depositor R Lane, RL Mellgren
Year of Origin 1990
References

Zhang W, et al. The major calpain isozymes are long-lived proteins. Design of an antisense strategy for calpain depletion in cultured cells. J. Biol. Chem. 271: 18825-18830, 1996. PubMed: 8702541

Basic Documentation
References

Zhang W, et al. The major calpain isozymes are long-lived proteins. Design of an antisense strategy for calpain depletion in cultured cells. J. Biol. Chem. 271: 18825-18830, 1996. PubMed: 8702541