CL37 (ATCC® CRL-2516™)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte

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These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody reacts with human E-selectin (endothelial leukocyte adhesion molecule 1 (ELAM-1)); CD62E. The antibody reacts, immunohistochemically, with rat pulmonary endothelial cells pretreated with tumor necrosis factor alpha (TNF-alpha).
Derivation
Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human E-selectin (endothelial leukocyte adhesion molecule 1 (ELAM-1)); CD62E.
Comments
The antibody reacts with human E-selectin (endothelial leukocyte adhesion molecule 1 (ELAM-1)); CD62E. The antibody reacts, immunohistochemically, with rat pulmonary endothelial cells pretreated with tumor necrosis factor alpha (TNF-alpha). CL37 antibody does not block in vitro adherence of neutrophils to TNF alpha-treated endothelial cells and the killing of TNF alpha-treated rat endothelial cells by phorbol ester activated neutrophils [PubMed: 1717513]. For antibody capable of blocking these activities see ATCC CRL-2515 (CL3).
Animals were immunized with human umbilical vein endothelial cells (HUVEC) that had been stimulated with recombinant interleukin-1 (IL-1).
Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(5) viable cells/ml. Maintain cell density between 5 X 10(4) and 1 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Culture Conditions
Temperature: 37.0°C
Isotype IgG1; kappa light chain
Name of Depositor C Smith
References

Mulligan MS, et al. Role of endothelial-leukocyte adhesion molecule 1 (ELAM-1) in neutrophil-mediated lung injury in rats. J. Clin. Invest. 88: 1396-1406, 1991. PubMed: 1717513

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS
References

Mulligan MS, et al. Role of endothelial-leukocyte adhesion molecule 1 (ELAM-1) in neutrophil-mediated lung injury in rats. J. Clin. Invest. 88: 1396-1406, 1991. PubMed: 1717513